TrypZean®

TrypZean® is a recombinant, animal-free alternative to bovine or porcine trypsin. TrypZean® is produced in plants and has the same kinetics as native trypsin [1]. TrypZean® performs similar to bovine or porcine trypsin in a number of applications [2] [3].

Purchase:

slimtrypsin
Code Description Size Price(USD)
TRL-001 TrypZean® lyophilized powder                                     (≥ 3350 USP units/mg solid) 1gram $2000



Discounted rates are available for larger quantities. Please contact our Product Manager by email at sales@appliedbiotech.org or by phone at 805-215-9500 for a personalized price quote.
For quantities less than 1 gram, visit Sigma-Aldrich (Product No. T3568).TrypZean® provides the following advantages:

  • Certified animal-free, TrypZean® eliminates the risk of animal-source contaminants such as viruses and bovine spongiform encephalopathy (BSE).
  • TrypZean® is free of contaminants associated with animal source trypsin and therefore can be used in lower concentrations in some applications [4].

Commerical applications include:

  • Cell culture: Dissociation of attachment –dependent cell lines from plasticware in serum-free and serum-supplemented systems
  • Insulin manufacture: Cleaves “C” peptide from proinsulin to form active insulin
  • Vaccine manufacture: Cleaves undesirable antigens from cells used to produce vaccines in both human and veterinary vaccine manufacture.

References:

1. Woodard, S. et al. Maize (Zea-mays)-derived bovine trypsin: characterization of the first large-scale, commercial protein product from transgenic plants. Biotechnology and Applied Biochemistry, 2003. 38(2): p. 123-120
2. Bielanski, A. et al. Prevention of bovine herpesvirus-1 transmission by the transfer of embryos disinfected with recombinant bovine trypsin. Theriogenology, 2013. 80(9): p. 1104-1108
3. Carvalho, P. et al. use of animal protein-free products for passaging adherent human adipose-derived stromal/stem cells. Cytotherapy, 2011. 13: 594-597.
4. Pau M., UytdeHaag, A. “Production of vaccines.” U.S. Patent No. 7,192,759, issued March 20, 2007.
5. Krishnan A, Woodard SL (2014) TrypZean™: An Animal-Free Alternative to Bovine Trypsin. Commercial Plant-Producesd Recombinant Protein Products- Case Studies (Howard JA, Hood EE, eds). Biotechnology in Agriculture and Forestry Volume 68, pp. 43- 63, Springer-Verlag, Berlin.
6. Salzig D, Schmiermund A, Grace PP, Elseberg C, Weber C, Czermak P (2013) Enzymatic Detachment of therapeutic mesenchymal stromal cells grown on glass carriers in a bioreactor. The Open Biomedical Engineering Journal 7:147-158

Specifications:
TrypZean®, bovine recombinant, expressed in Zea mays
Storage temperature: 2-8˚C

Test Specification
Appearance White to off-white powder
Enzymatic Activity
USP units/mg solid
BAEE units/mg solid

≥ 3,350 USP
≥ 10,950 BAEE
Purity by SDS Electrophoresis
Banding corresponds to 24-28 kDa
≥ 90 %

FAQ:
1. What is Trypsin?
Trypsin is a protease derived from bovine or porcine pancreas that is used to digest other proteins. It specifically cleaves peptide bonds after basic amino acids such as lysine and arginine.
2. How is it used?
There are many market segments that use trypsin including bioprocessing, commercial cell culture, wound care, foods, and digestive aid.  It is used in the manufacture of several pharmaceutical proteins including insulin.
3. Is TrypZean® certified animal-free?
Yes.
4. How can I order TrypZean®?
You can purchase TrypZean® directly from the Applied Biotechnology Website or from Sigma-Aldrich (Product No. T3568). If you would like to purchase more than 1 gram, please contact our Product Manager for TrypZean® for a personalized price quote by email at sales@appliedbiotech.org or by phone at 805-215-9500.
5. Do you provide a Certificate of Analysis?
Yes, each lot of TrypZean® comes with a Certificate of Analysis.

Material Safety Data Sheet (MSDS)
TrypZean® Material Safety Data Sheet