TrypZean® is an animal-free source of recombinant bovine trypsin expressed in maize . Trypsin is a serine protease produced by the pancreas. It catalyzes the degradation of protein by specifically cleaving next to lysine and arginine.
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Avidin is a glycoprotein that binds tightly to biotin. It is used as an industrial reagent, most notably in diagnostics kits. ABI offers a recombinant avidin, certified animal-free, eliminating the risk of pathogens.
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Cellobiohydrolase is a β-glucanase, CBHI, from Hypocrea jecorina, expressed in maize [2-3]. CBHI is an exo-cellulase enzyme which cleaves cellulose at non-reducing ends of the polymer, yielding cellobiose. Cellobiohydrolase can be used in combination with endocellulases and beta-glucosidase to produce glucose from cellulose. Its activity is dependent on several factors including inhibitors in the substrate.
Purchase: Cellobiohydrolase can be obtained from Sigma-Aldrich (Product No. E6412) or by contacting Infinite Enzyme directly at www.infiniteenzymes.com.
Endocellulase is an endoglucanase, E1, from Acidothermus cellulolyticus, expressed in maize [2-3]. The enzyme is highly specific to cleaving the 1,4-glycosidic bond of the cellulosic chain. This can be used to treat pulp fibers to increase their self-adherence when making paper sheets. Enzyme preparations can also be used in combination with cellobiohydralase and β-glucosidase to produce glucose from cellulose.
Purchase: Endocellulase can be obtained from Infinite Enzyme directly at www.infiniteenzymes.com.
1. Woodard, S. et al. Maize (Zea mays)-derived bovine trypsin: characterization of the first large-scale, commercial protein product from transgenic plants. Biotechnology and Applied Biochemistry, 2003. 38 (2): p. 123-130.
2. Hood, E. et al. Subcellular targeting is a key condition for high-level accumulation of cellulase protein in transgenic maize seed. Plant Biotechnology Journal, 2007. 5 (6): p. 709-719.
3. Hood, E.E. et al., Manipulating corn germplasm to increase recombinant protein accumulation. Plant Biotechnology Journal, 2012. 10 (1): p. 20-30.
4. Hood, E. et al. Commercial production of avidin from transgenic maize: characterization of transformant, production, processing, extraction, and purification. Molecular Breeding, 1997. 3 (4): p. 291-306.