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Licensing
ABI is eager to hear proposals for collaborations using
transgenic plants. In addition to any new projects, ABI welcomes
discussion on the collaboration of its existing projects or the
licensing of proprietary technology. Some of the available
options are listed below. For more information, contact
jhoward@appliedbiotech.org
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Available Licenses |
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Technology |
Function
|
Utility |
Description |
|
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Constitutive promoter |
Drive expression in different plant tissues |
Useful to drive selectable markers, agronomic traits,
and output traits. |
Demonstrated expression of various
proteins in leaves and seeds of maize. May work in other
plants as well.
Streafield et
al, 2004 |
|
|
Embryo promoters |
Tissue preferred expression in embryo |
Confer expression of proteins that are
targeted specifically to the embryo with little or no
expression in other tissues. May be useful to
confer high oil traits, seed traits or over expression
of industrial or pharmaceutical proteins. |
Demonstrated high expression of
proteins in maize germ. May work in other plants as
well.
Streatfield et al, in prep |
|
|
OPH marker |
Selectable or scoreable marker |
Useful to identify plant transformation events. |
Demonstrated to work in maize cultures and plants. May work in other plants as well.
Pinkerton, in press |
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Collaborations |
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|
Project |
Technology
|
Utility |
Reference |
|
|
Biofuels/Ethanol |
Several hydrolytic enzymes including endo and exo
cellulases over expressed in maize |
The break down of cellulose biomass requires low cost
and large volumes of enzymes. This technology can
potential provide the supply of enzymes |
Demonstrated high expression of
active proteins.
Hood et al,
in press
Howard, in
press
Clough et al, 2005
Bailey et al, 2003
Hood, et al 2003 |
|
|
Digestive Enzymes |
Hydrolytic enzymes used in the deconstruc- tion of plant
cell walls |
Providing hydrolytic enzymes can allow for greater
digestion of food and feed in animals. This technology
allows for enzymes to be introduced directly into the
food or feedstock thereby allowing the potential for
much greater doses of enzymes at lower costs.
|
Demonstrated high expression of
active proteins.
Hood et al,
in press
Clough et al, 2005
Bailey et al, 2003
Hood et al, 2003 |
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|
Oral Vaccines |
HepB, HIV, LT-B, TGEV antigens expressed in maize |
The plant-expressed antigens potentially provide for a
method of orally-delivered vaccines in animals at low
cost. |
Demonstrated production of
antibodies and protection by oral delivery.
Lamphear et al.
2004 Tacket
et al. 2004
Streatfield, et al 2003a
Streatfield et al, 2003b
Streatfield et al, 2003c
Lamphear et al.
2002
Streatfield et al, 2001 |
|
|
Pharmaceutical for wound healing |
Aprotinin |
A non-animal source of this current pharmaceutical can
be produced avoiding issues around animal contaminants
such as Mad Cow Disease. |
Demonstrated functional and chemically equivalence with
protein isolated from native host.
Delaney et al.
2003 Zhong
et al, 1999 |
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|
High intensity natural sweetener |
Brazzein |
A natural and relatively stable sweetener for foods |
Demonstrated expression and
sweetener activity in plants.
Lamphear et al.
2004 |
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References
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Delaney D, J Jilka, D Barker, P Irwin, M Poage, S
Woodard, M Horn, A Vinas, K Beifuss, M Barker, B
Wiggins, C Drees, R Harkey, Z Nikolov, E Hood, J
Howard. 2003. Production of aprotinin in transgenic
maize seeds for the pharmaceutical and cell culture
markets. In Plant Biotechnology 2002 and Beyond,
pp.393-394. Proceedings of the 10th IAPTC&B Congress,
Orlando, Florida. IK Vasil (ed.). Kluwer Academic
Publishers, The Netherlands.
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Streatfield SJ, JR Lane,
CA Brooks, DK Barker, ML Poage, JM Mayor, BJ Lamphear,
CF Drees, JM Jilka, E. Hood and JA.Howard. 2003c.
Corn as a production system for human and animal
vaccines.Vaccine 21: 812-815.
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Hood EE, MR Bailey, K Beifuss, M Horn, M
Magallanes-Lundback, C Drees, DE Delaney, R Clough and J
A Howard. 2003. Criteria for high-level expression of a
fungal laccase gene in transgenic maize. Plant
Biotechnology. J. 1:129-140.
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Bailey
MR, SL Woodard, E Callaway, K Beifuss, D Delaney, M
Magallanes-Lundback, J Lane, ME Horn, M Ward, F Van Gastel,
JA Howard, EE Hood. 2003. Improved recovery of active
recombinant laccase from maize seed. Appl. Microbiol.
Biotechnol. 63(4): 390-397
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Lamphear BJ, SJ Streatfield, JM Jilka, CA Brooks, DK
Barker, DD Turner, DE Delaney, M Garcia, B Wiggins, SL
Woodard, EE Hood, IR Tizard, B Lawhorn, JA Howard.
2002. Delivery of subunit vaccines in maize seed. J.
Control. Release 85:169-180.
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Streatfield SJ, JM Jilka, EE Hood, DD Turner, MR
Bailey, JM Mayor, SL Woodard, KK Beifuss, ME Horn, DD
Delaney, IR Tizard, JA Howard. 2001. Plant-based
vaccines: unique advantages. Vaccine 19: 2742-2748.
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Zhong
G-Y, D Peterson, DE Delaney, M Bailey, DR Witcher, JC
Register III, D Bond, C-P Lin, L Marshall, E Kulisek, D
Ritland, T Meyer, EE Hood, JA Howard. 1999. Commercial
production of aprotinin in transgenic maize seeds.
Molecular Breeding 5: 345-356.
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